April 15, 2018 Weekly goals

So last week was unfortunately not as productive as I had planned as a result of some dog-sitting shenanigans. So, to help myself get back on top of things, I’m making a list of what I want to and will accomplish this week. Firstly, tomorrow I have a presentation in my FISH 511 class, so I will be focusing on that today and tomorrow. The rest of the week will be as follows:

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Dia Analysis Re Do

I re-did the DIA analysis from the beginning. This time I only used settings used by Emma in her Skyline document that she sent me.

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Updates Podcast Dia

Podcast

Today Steven and I recorded a little bit of us talking about the spreadsheet and how I’ll be doing the RNA isolating. What I am going to work on is an episode, or several short episodes, about the background of the project: bitter crab disease; Tanner crabs; Juneau, etc. I also am going to make a quick little intro that we can put at the beginning of all the episodes. I want to make one weekly, or at least release one weekly. And I would like to have some way to make it more easily understandable to people like my mom, or my friends. To do that I think I just have to make sure I explain what I’m talking about more. Obvoiusly I shouldn’t be putting too much time into it because the main focus is on the research itself, but I’m excited and looking forward to learning more about this outreach tool and communication strategy.

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March Goals

March Goals

  • Isolate crab hemolymph RNA
  • Weekly Podcasts
  • DIA on 2015 Oysterseed experiment (make some headway on this)
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Crab Mtg Number1

Today we had our first crab project meeting. Our plan is to meet the first Thursday of the month (subject to change if need be).

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Rna Isolation Mistake

Today I began the process of isolating RNA from the “official” crab hemolymph samples, although they can change if I mess things up, because there are many extra samples.

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Thesis Proposal Writing

This quarter I am taking FISH 521, which is the Research Proposal Writing and Professional Development course taught by Julian Olden. This class has been very helpful because it has guided me through the layout of the Crab Project and the plan of how it will all be performed. This is good becuase this is my first quarter of graduate school and I had only heard about the crab project around Thanksgiving of 2017.

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Rna Isolation Podcast Thoughts

RNA isolation

I am either blind or we don’t have it, but I could not find RNAzol RT. I found all the other reagents needed, but not the RNAzol. I thought I saw it, but it was DNAzol. So the RNA isolation has to wait until either someone proves me wrong and finds it, or we order more.

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Rna Isolation Protocol For Tomorrow

Tommorrow I will be trying out RNA isolation on 2 extra Tanner crab hemolymph samples. Try protocol with 2 samples: 465 and 468 Both samples are yellow (from third sampling day) and are from crabs that are infected, immature, and held at ambient temperatures throughout the experiment.

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Samples For Rna Extraction

Today I re-picked the samples that I’ll use for RNA extraction. I realized that I could easily choose samples from the same crabs across all sampling days and temperatures. I went back and amended the initial post with the new choices, so that I don’t get confused in the future.

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Podcast

I tested out the USB Microhpone Samson Meteor Mic (2016). It connects to my laptop via a USB cord. I opened up GarageBand and started a new Audio/Voice Project. There are many different vocal effects options, but I just used the basic “Narration vocal”. Press the red circle record button to begin, and press again to stop. After you are done recording, you can edit the vocal recording.

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February Goals

I would have made this post on the actual first day of February, but I have been sick - the neverending cold. Anyway, for this short month I have some goals I’d like to achieve:

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Crab Sample Picking

Today I chose some crab samples for RNA extraction. I made a chart with the tube numbers for the samples. All crabs are immature males. Due to a massive die-off in the warm temperature treatment groups, we unfortunately only have two infected and one uninfected sample per the final two sample days. As a result, the uninfected samples will not be “pooled”.

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Qpcr Training

Today the new qPCR machine arrived. Brian showed us the basics of using the machine and the software. He offered to come back anytime once we start using the machine to help answer questions specific to our project.

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2015oysterseed Dia Spot Check

I moved computers from Swan to Woodpecker. In order to be able to continue a project on a different computer, you need to File > Share > Complete. It becomes a .zip file. Upload to OWL. Open zip on new computer, and all the libraries and everything is included.

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