Re-running plate 4 samples to see if things improve. Spoiler: they didn’t. Details in post.
qPCR Plate 5 Info:
Plate Map 5: here (plate is re-run of same samples from plate 4).
Plate 5 qPCR Run Results:
| qPCR plate 5 | |
|---|---|
| R2 | 0.928 |
| E | 139.90% |
| Y-int | 30.583 |
| Slope | -2.632 |
Result files live in this google drive: here
Feedback on how to move forward
See this GitHub Issue: #2487
Waiting to hear on what the best course of action is, since the efficiency is still too high, rendering the results unusable :(.
Notes:
- Will need to order more TaqMan Master Mix (currently have 1 full tube and one half tube ~ enough for 1 full plate).