See post for details!

JULY GOALS: Multi-Species

RNA Extractions and RNA Send Off

Finish RNA extractions for Day 12 controls and Day 6 Exposed and Control. Total RNA samples to send –> n=54.

THe quote and everything is all accepted, just need to finish extractions, run samples on Qubit to ensure there’s RNA (if not, then I’ll have to select other samples) and then send off.

Multispecies Analyses

P. helianthoides

The alignment of the 6 RNAseq libraries from the 6 P. helianthoides exposed stars from day 12 are aligned to the P. helianthoides genome. I have gene count and transcript count matrices.

P. ochraceus

I contacted Mike and Lauren and got help figuring out how to make the files I had correlate to each other in order to complete alignment to the P. ochraceus genome. Got that all sorted and now have matrices, though I still want to go through and ensure they’re correct-looking.

D. imbricata

I contacted Mike and Lauren for this dataset as well, and they found a whole bunch for files to send me with the help of another collaborator of theirs, Melissa. I’m about to start working through what these files are and how they correlate to each other in order to complete alignment for the D. imbricata libraries.

Each Species work to do:

  1. Align n=6 libraries to respective genome
  2. Characterize genes/transcripts expressed

What else?? Should I also assmeble transcriptomes? Chat about this with Steven in our meeting this week.

WDA Presentation

Finish characterizing genes expressed in each species at time point Day 12 in the exposed stars.

Make figures:

  1. Disease signs over time (have this already)
    What other figures? Ask Steven for ideas.