Going through RNA Extraction protocol with 4 samples before doing batches of 18 samples to re-familiarize self with the process. See post for details.

Samples chosen

Sample ID	Species	Sample Day
312	D. imbricata	0
319	P. ochraceus	0
326	P. helianthoides	0
336	D. imbricata	0

RNA Extraction protocol

Following manufacturer’s protocol for Zymo Research Quick DNA/RNA Microprep Plus Kit (Cat D7005).

Label tubes and everything is set up by 1:30pm (started around 1pm).

Finished extraction of the four samples at 3;15pm.

I put the extracted RNA in the brown box in the bottom shelf of the left -80C. Not in a rack or tray.

Tomorrow:

Extract RNA from 18 samples and then run the 18 + 4 from today on Qubit.

The samples I need to get RNA from (n=54) to then send off for RNAseq are below:

experimental_day	star_ID	species	treatment	coelomocyte_ID_PSC
6	B1_D105	dermasterias_imbricata	exposed	421
6	B1_Pi120	pisaster_ochraceus	exposed	422
6	B1_Py89	pycnopodia_helianthoides	exposed	423
6	B10_D113	dermasterias_imbricata	control	430
6	B10_Pi127	pisaster_ochraceus	control	431
6	B10_Py86	pycnopodia_helianthoides	control	432
6	B11_D114	dermasterias_imbricata	control	436
6	B11_Pi130	pisaster_ochraceus	control	437
6	B11_Py81	pycnopodia_helianthoides	control	438
6	B12_D110	dermasterias_imbricata	control	442
6	B12_Pi132	pisaster_ochraceus	control	443
6	B12_Py85	pycnopodia_helianthoides	control	444
6	B14_D103	dermasterias_imbricata	control	454
6	B14_Pi123	pisaster_ochraceus	control	455
6	B14_Py94	pycnopodia_helianthoides	control	456
6	B16_D98	dermasterias_imbricata	control	466
6	B16_Pi126	pisaster_ochraceus	control	467
6	B16_Py95	pycnopodia_helianthoides	control	468
6	B2_D104	dermasterias_imbricata	exposed	427
6	B2_Pi128	pisaster_ochraceus	exposed	428
6	B2_Py83	pycnopodia_helianthoides	exposed	429
6	B3_D100	dermasterias_imbricata	exposed	433
6	B3_Pi131	pisaster_ochraceus	exposed	434
6	B3_Py91	pycnopodia_helianthoides	exposed	435
6	B4_D106	dermasterias_imbricata	exposed	439
6	B4_Pi121	pisaster_ochraceus	exposed	440
6	B4_Py84	pycnopodia_helianthoides	exposed	441
6	B6_D101	dermasterias_imbricata	exposed	451
6	B6_PI118	pisaster_ochraceus	exposed	452
6	B6_Py86	pycnopodia_helianthoides	exposed	453
6	B8_D111	dermasterias_imbricata	exposed	463
6	B8_Pi119	pisaster_ochraceus	exposed	464
6	B8_Py26	pycnopodia_helianthoides	exposed	465
6	B9_D107	dermasterias_imbricata	control	424
6	B9_Pi124	pisaster_ochraceus	control	425
6	B9_Py92	pycnopodia_helianthoides	control	426
12	B10_D113	dermasterias_imbricata	control	526
12	B10_Pi127	pisaster_ochraceus	control	527
12	B10_Py86	pycnopodia_helianthoides	control	528
12	B11_D114	dermasterias_imbricata	control	532
12	B11_Pi130	pisaster_ochraceus	control	533
12	B11_Py81	pycnopodia_helianthoides	control	534
12	B12_D110	dermasterias_imbricata	control	538
12	B12_Pi132	pisaster_ochraceus	control	539
12	B12_Py85	pycnopodia_helianthoides	control	540
12	B14_D103	dermasterias_imbricata	control	550
12	B14_Pi123	pisaster_ochraceus	control	551
12	B14_Py94	pycnopodia_helianthoides	control	552
12	B16_D98	dermasterias_imbricata	control	562
12	B16_Pi126	pisaster_ochraceus	control	563
12	B16_Py95	pycnopodia_helianthoides	control	564
12	B9_D107	dermasterias_imbricata	control	520
12	B9_Pi124	pisaster_ochraceus	control	521
12	B9_Py92	pycnopodia_helianthoides	control	522