Today I played around with GitHub and R Projects. I’ve figured out a way of making adding Qubit results to my hemolymph and Qubit results master file more easy. It isn’t perfect and there are likely some things that I don’t know about that could make it easier, but I’ve detailed the new method below. I also noticed today that the tube_number column in what I did yesterday got all messed up with the merge in R. Detailed below.
New R Project and Script
Sam mentioned yesterday that it was difficult to help me because my R script and project was traversing two GitHub repositories.
So, today I worked on figuring out a way to make it easier not only for myself, but for future collaborations and help.
The script is much more straightforward and easier to use becuase the csv’s are already in this repository.
library(tidyverse) # Script for creating joined and merged data files from project-crab. Ultimate goal is to create a true master file of all crab-related data files in this repo. # Join all hemolymph sampling data with Qubit RNA HS results data into one file. # read in all-crabs-hemo.csv hemo <- read.csv("data/20180522-all-crabs-hemo.csv") # read in Qubit-results.csv qub <- read.csv("data/20180817-Qubit-results.csv") # Convert "tube_number" in hemo.csv numeric (not factor) hemo$tube_number <- as.numeric(hemo$tube_number) # test that as.numeric conversion worked is.numeric(hemo$tube_number) # Join files based on column "tube_number" master <- left_join(hemo, qub, by = "tube_number") # Look at columns in master file colnames(master) # Select columns that are useful and relevant master2 <- master %>% select(year.FRP, FRP, infection_status, Uniq_ID, sample_day, infection_status, maturity, Holding_Tank, Low_Tag, High_Tag, sample, treatment_tank, where, tube_number, Comments, death, comments, CW, SC, CH, Test_Date, Original_sample_conc_ng.ul, total_sample_vol_ul, total_yield_ng) %>% arrange(FRP) # Write updated csv to project-crab data repo. write.csv(master2, "20180817-hemo-Qubit.csv") # Move new csv to data directory in repo project-crab and update readme.md
With this current process there are some things that I need to do outside of R. I add the new Qubit data to the 20180817-Qubit-results.csv file and add in the “total_sample_vol_ul” and the “total_yield_ng” values. Then, at the end of the script, I have to move the new csv to the data directory within the project-crab repository and update the readme.md.
Merge in R messed up tube_number columns
```#Try to merge columns that are repeats. “Test_Date.x” with “Test_Date.y” and “Original_sample_conc_ng.ul.x” with “Original_sample_conc_ng.ul.y”.
qSam <- read.csv(“20180813-results_from-Sam.csv”) hemoqub <- read.csv(“../project-crab/data/20180813-all-hemo-with-Qubit.csv”)
updated <- merge(x = hemoqub, y = qSam, by = c(“tube_number”, “Test_Date”, “Original_sample_conc_ng.ul”), all.x = TRUE)
write.csv(updated, “20180816-all-hemo-with-Qubit.csv”) ```
Resulted in the new file’s columns getting messed up.
20180816-all-hemo-with-Qubit.csv The tube numbers are in column marked “X” now…