Rna Isolation And Dia Continued

RNA Isolation

I isolated RNA from 9 more samples: img

Note that sample 305 is actually labeled as “#4”.

During the isolation, tubes 66 and 26 (infected, first day samples) I wasn’t able to see any obvious pellets like I have been able to in all other tubes.

DIA

I am in the process of checking the error rate. A lot of them look good.

Below is an example of one that I’m not 100% sure about, but I am giving it a “0” score because the peak boundaries are off and becuase the peaks are slightly different. So, currently, I am only giving “1” scores to perfect peptides across all samples. Not sure if this is reasonable. img

Written on March 21, 2018