See post for details on what I want to touch base on with Steven tomorrow!

Updates:

  • Multispecies RNA extractions are going great!! –> I’ll be finishing the rest tomorrow and then sending out to seq facility on Wednesday morning
  • I think I now have all the files I need for aligning the current multispecies dataset to their respective genomes - namely the D. imbricata and P. ochraceus files

What I’d like guidance on:

What’s the best way to go about describing and comparing the different gene expression profiles for the current dataset so I can share at Wildlife Disease Association Conference (July 27-Aug 1).

I’m thinking:

  1. Align RNAseq data to respective genomes
  2. Annotate with whatever annotation files I have from the collaborators
  3. BLAST?
  4. Gene ontology annotation

What else?

I’m also trying to think of interesting visuals - but I think that will come up in next week’s meeting with Steven because the first step is finalizing the alignments for D. imbricata and P. ochraceus