Sam extracted RNA using the Quick DNA/RNA Microprep Plus Kit (ZymoResearch) (post). I don’t think there is currently enough RNA in the libraries to reach the requiremets for NWGC… details in post.
Today I quickly joined his qubit result file with the hemolymph sampling data (hemo_table.csv) in R.
The resulting file: hemo_qub-for-libs.csv
The goal is to have 12 libraries accounting for each infection status and temperature treatment. Each pooled library needs to have at least 1000 ng of RNA in a sample of ~50ul. (GitHub Issue #184)
Pooled libraries to create:
|Library Number||Sample Day||Infection status||Temp trtmnt|
*Note: the temperature treatments didn’t start until after the Day 9 sampling day.
As of now, I don’t think the pooled samples would have enough RNA to meet the requirements… for example, the samples that fall under Library 1 add up to 79.9 ng/ul.
I may need to check this again when I’m not sick.