Submitted Thesis Chapters to Committee; Start working with New transcriptome
Today I submitted my two thesis chapters to my committee, and between now and June 2nd (my defense date), I’ll be doing more differential expression analyses with the new transcriptome (not filtered based on taxonomy). Plan for the work and what I’ve done so far detailed in post.
I’ll also write up a thesis introduction that ties the two chapters together.
New transcriptome work so far:
I copied over the methods and results from the first crab paper (the one I sent to my committee), and am replacing the statistics, numbers, etc. with those from the new transcriptome. I also made a new stress response gene list and a new transcriptome GOslinm pie chart:
Jupyter notebook where I took the new
blast results to GOslim: here
blast results: here
New transcriptome Blastquery-GOslim.tab
I also made a table of all the samples that we have RNAseq data for (in paper). I made it based on this google sheet (tab labeled “table-for-paper”). I also used this google sheet (here) to figure out the individual crabs and come up with a crab ID scheme. Currently am using letters A through I (9 crabs, 6 have 3 time points of RNAseq data, 3 have 2 time points (the warm crabs that didn’t survive the experiment)).
New plan between now and June 2nd
- Next week –> differential expression analyses (details will be discussed 05/14 on specific comparisons)
- Week after –> modify paper (intro, discussion) based on new results
I’ll also be doing a practice “talk” on May 22nd… won’t be a full on talk, will just be getting feedback on flow of presentation and ideas on what is important and isn’t to include in defense.