Lab Notebook

I sent a .sh to Mox to perform a BLAST with my assembled C. bairdi transcriptome and swiss prot. I am also working on figuring out how to do this on Hummingbird (on toaster/grace). Additionally, I have come up with a list of things I want to accomplish this week!... [Read More]

Trinity My Trinity job failed and it turns out it’s because I was missing a \ after /gscratch/srlab/programs/Trinity-v2.8.3/Trinity. I fixed that and then re-sent the job to Mox. The new script is called 20181101_Cbairdi_trinity.sh and it lives in my /gscratch/srlab/graceac9/jobs/ directory on Mox. [Read More]

Crab Project RNA extractions and Libraries (Get this done before I go home Dec 21st) Come up with RNA extraction plan for the remaining libraries Perform extractions using Qiagen RNeasy Plus Micro Kit Data organization Create new R scripts for adding new Qubit results data to a master file combining... [Read More]

Today I ran BLAST with the bad fasta from the Trinity run from last weekend. Will look more at the notebook Steven sent me to do the BLAST stats, goslim, contigs, go slim tables, etc. I also have been getting some input from Sam as to how best to manage... [Read More]

Today I re-ran Trinity because the original files I used that I downloaded to my computer from nightingales were too small and not .fastq.gz. Sam showed me how to do rsync from nightingales to mox. I also am working on doing a BLAST with the finished Trinity.fasta that is too... [Read More]