Ran the last remaining eelgrass swab DNA from FHL 2026 on qPCR targeting Vibrio pectenicida. See post for details.

qPCR Plate 9 Info

Plate Map: samples here

      Each Mix
Ingredient   per 20ul rxn 72
Mix   10 792
10X Exo IPC Mix   2 158
50 Exo IPC DNA   0.4 32
VPT4-F (10 um)   0.2 16
VpT4-R (10 um)   0.2 16
Probe (10 um)   0.2 16
H20   4.5 356
BSA   0.5 40
Template   2  
Total   20 1426

Plate 9 qPCR Run Results:

here

img

Notes:

I need to do the following things before I can run another plate:

  • Redilute more of the standard curve
  • Re-aliquot/dilute the forward and reverse primers
  • Re-aliquot/dilute the probe
  • THERE ARE NO MORE P10 TIPS!!! I don’t have any funding… So I’ll need to ask Steven if there are lab funds for lab supplies for everyone…. ?

Results plots

See current results post!