See post for details on extracting DNA from eelgrass swabs from the FHL 2026 V. pec., eelgrass and mussel experiment - PART 2!

Background info on the project

Post: FHL 2026 Experiment Details

DNA Extraction of Eelgrass Swabs Info:

Samples processed

sample_id treatment_id treatment sample_type
S_ESW_03 ESW eelgrass + sw eelgrass_swab
S_ESW_05 ESW eelgrass + sw eelgrass_swab
S_ESW_06 ESW eelgrass + sw eelgrass_swab
S_EVP_04 EVP eelgrass + vp eelgrass_swab
S_EVP_05 EVP eelgrass + vp eelgrass_swab
S_EVP_06 EVP eelgrass + vp eelgrass_swab
S_EVP_07 EVP eelgrass + vp eelgrass_swab
S_EM_01 EM eelgrass + mussel + sw eelgrass_swab
S_EM_05 EM eelgrass + mussel + sw eelgrass_swab
S_EM_06 EM eelgrass + mussel + sw eelgrass_swab
S_EMVP_05 EMVP eelgrass + mussel + vp eelgrass_swab
S_EMVP_06 EMVP eelgrass + mussel + vp eelgrass_swab
S_EMVP_07 EMVP eelgrass + mussel + vp eelgrass_swab
S_EMVP_08 EMVP eelgrass + mussel + vp eelgrass_swab
S_Blank_03 NA NA NA
S_Blank_04 NA NA NA

Protocol notes:

Extraction Kit: Qiagen DNeasy PowerSoil Pro Kit (Cat: 47014)

Extraction Notes: here

Protocol was modified by Becca Maher specifically for swabs from eelgrass tissue (see link to notes above).

Eelgrass swab sampling protocol: here

Results

Stay tuned for when I run 2ul of each sample on qPCR targetting V. pec!