No enrichment with shared orthogroups as the background and Day 12 DEG uique-to-species orthogroups as the gene lists.

There isn’t enrichment for Day 12 with the shared orthogroups as the background. No results from DAVID.

Code files:
project-pycno-multispecies-2023/code/28-orthogroup-annot.Rmd: To annotate the shared orthogroups.

project-pycno-multispecies-2023/code/29-DAVID.Rmd: To create lists of uniprot accession IDs for the input for DAVID.

Input files were these:

Dermasterias imbricata unique orthogroup Uniprot Accession IDs from Day 12 DEGs

Pisaster ochraceus unique orthogroup Uniprot Accession IDs from Day 12 DEGs

Pycnopodia helianthoides unique orthogroup Uniprot Accession IDs from Day 12 DEGs

Background file:

Uniprot accession IDs from the orthogroups shared across all three species: output/28-orthogroup-annot/shared_orthogroups_DAVID_background.tab

Results:

No enrichment for any of the three species’ Day 12 DEGs with the shared orthogroups as the background.