Today I ran 2ul of the 24 samples I extracted yesterday. 20 out of 24 had RNA. Details in post.
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Notes on protocol and plan for Crab Project extractions - use info from Sam's notebook posts
This post contains my notes on the Zymo Research Protocol for Quick-DNA/RNA Microprep kit, and Sam’s notebook post to create a protocol for extracting RNA. I also include my extraction plan for this next week as I try to get enough RNA to get two more libraries (Day 9 infected...
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October 2019 Goals
Crab
Work on paper
Pick samples accounting for temperature and infection status from master-qubit-sample.csv
Assemble transcriptomes, annotate etc. once new data arrives (should be any day now!)
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Oysterseed DIA - trying to characterize list of detected proteins in relation to _C. gigas_ proteome
I’m still working on wrapping up the results and discussion of this paper. Steven recommended I look into characterizing the list of detected proteins (2,808) in relation to the whole C. gigas proteome. Detailed in post:
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Oysterseed DIA - New Analyses and wrapping up paper
I’ve been working on finishing up the Oysterseed DIA paper, and met with Steven today to discuss it. Details in post below:
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