Oyster Paper
Finish solid draft by 4/2 or 4/3 at the latest and send out to lab for feedback
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Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the Zymo Microprep kit
So, it turns out my 6 pooled samples aren’t concentrated enough for NWGC to sequence. They are all at around 25ng/ul, but they need to be at least 50ng/ul. I called zymo yesterday to find out how best to do that. They recommended a kit (free sample coming next week)....
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December 2019 Goals
Crab:
Submit 6 new pooled samples to NWGC (waiting to hear back about how to submit samples (GitHub Issue: #798))
Work on assembling transcriptomes and moving forward with other 4 pooled libraries we currently have
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Pooled 6 new samples for sequencing
Today I pooled samples for the 6 new libraries for sequencing. There is over 1000ng in each pooled sample, so everything is ready to go! Details in post.
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More RNA extracted from Day 9 - RNA in all
I extracted RNA from the same tubes that I worked with a lot the past couple of weeks on the bioanalyzer and Nanodrop and qubit because there ended up being not much left. RNA was in all samples. Details of extraction and next steps in post.
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