I forgot to do March goals at the beginning of the month… so here’s what my goals are for the rest of the month! Classes are over this Friday, and I’m not going anywhere for the break, so I think I can get this done!
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RNeasy Kit Tube 516-2 starting sample testing
Today I extracted RNA from tube 516-2 in two different volumes: 10ul and 50ul. The tube only had about 62ul of sample slurry remaining, so I was only able to do 10ul and 50ul. I used the same protocol as what Steven and Shelly did last Friday. I saved the...
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RNeasy Kit with hemo pellets using QIAshredder columns
Today, Steven, Shelly, and I worked together on extracting RNA from bairdi hemolymph pellets using the Qiagen RNeasy Micro Plus Kit with QIAshredder columns to aid in homogenization. Instead of using the whole pelleted sample like I have always done in the past, we let it thaw at room temp,...
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Supernatant Trizol LS RNA Extraction attempt
After talking with Steven and Shelly, today we decided I should attempt to extract RNA from supernatant samples using the Trizol LS protocol. This post contains the steps that I performed with pictures of some steps, and results (no RNA in final samples :(…). Overall current plan is to take...
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RNeasy Plus Micro Kit Protocol using RNA Carrier and Supernatant
This post contains a slightly modified RNeasy Kit protocol. I want to use the Qiagen RNeasy Micro Plus Kit on 4 crab hemolymph pellet samples and the four corresponding supernatant samples (GitHub Issue 577). The goal is to see if there is any RNA in the supernatant samples and if...
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