Crab Meeting today: shared current status of what we’ve got (FISH546 grace-Cbairdi-transcriptome). Additionally, we had a proteomics meeting today and I got some input on next steps for the DIA: set some stringent thresholds, MS Stats, make some tables, etc. I will make a separate post on what I did...
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Make Taxonomy Pie Charts in R
Today I fiured out how to make pie charts in R, and I did so with the taxonomy output from the BLAST with the C. bairdi transcriptome and the nt taxonomy database. I mostly worked on my repo for Fish546, and it’s still a work in progress. Also, update on...
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Start new DIA analysis using Walnut, EncyclopeDIA, and Bri-line
Today I tried doing the mprophet model in Skyline Daily. It wasn’t super great and I got stuck, but Emma reminded me that a lot of people who do DIA analysis prefer a new method using Walnut, EncyclopeDIA, and Bri-line. I started this new protocol today and am currently letting...
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Cold, Infected Pool with RNeasy and DecaPod S1E13
Today I edited and published S1E13 of DecaPod. In this episode I interview Madi Shipley (MS, SAFS) who works with Bering Sea Tanner crabs. I also extracted RNA from 8 samples (all Cold, infected, Day 26) using Qiagen RNeasy and definitely have enough RNA for a pool!
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RNeasy Test Bioanalyzer Results
Today I ran the two samples from Day 26 that I extracted using RNeasy Kit on the Bioanalzyer. The results look pretty good and we can now make a library and see how it goes.
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