See post for details for qPCR plate #8.

Samples run:

I pooled DNA into three pools:

Pool 1 –> 1ul of DNA from each (n=16) of the exposed eelgrass swab DNA samples. These came from treatment groups that were exposed to V. pectenicida.

Pool 2 –> 1ul of DNA from each (n=16) of the control eelgrass swab DNA samples. These came from treatment groups that were NOT exposed to V. pectenicida.

Pool 3 –> 2ul from each (n=6) of the DNA extraction blanks from the eelgrass swab DNA extractions. These should not have any V. pectenicida DNA in them.

Plate run:

  1. Standard curve
  2. NTC
  3. The three pools

Each in triplicate.

Plate map: here

Results:

img

Result Files: here.

Thoughts

Sam added thoughts to this GitHub Issue: here