Today I submitted 6 pooled samples of Crab RNA to NWGC for QC. After they run QC, they’ll let us know what our sequencing options are.

GitHub Issue: #798

Pooled the samples originally on Nov 22nd, 2019

After pooling, I realized I had to have samples concentrated to NWGC minimum requirement of 50 ng/ul… so I attempted to concentrate them using a kit from Zymo after it arrived in the mail.

Attempt at concentrating was unsuccessful becuase I didn’t do the correct volume of RNA Binding Buffer (post: here).

Sam recommended I contact NWGC and ask if we can still get some sequencing done even though our samples are not at their requirements, which brought us to today!

I put the 6 pooled samples in a plate that was provided by Jeff Weiss at NWGC, and walked them over there.

They will do QC testing, and then let us know what our sequencing options are afterwards.

Here’s manifest of what I submitted today:

Plate Well Location Investigator Sample ID Additional Sample ID Family Number Replacement Sex Date of Birth Organism Race Concentration (ng/uL) Volume (uL) RNA Quality Score Sample Source Type of Sample Suspended In Extraction Method Certified for dbGaP dbGaP ID Investigator Last Name
  (Required)       (Required)   (Required)   (Required) (Required) (Required if RNAseq)   (Required) (Required)       (Required)
A:1 D9_0       Male   Chionoecetes bairdi   15.7 33   crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit     Crandall
B:1 D9_1       Male   Chionoecetes bairdi   17.6 33   crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit     Crandall
C:1 D12_cold_0       Male   Chionoecetes bairdi   24.9 33   crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit     Crandall
D:1 D12_cold_1       Male   Chionoecetes bairdi   26 33   crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit     Crandall
E:1 D12_warm_0       Male   Chionoecetes bairdi   26 33   crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit     Crandall
F:1 D12_warm_1       Male   Chionoecetes bairdi   24.4 33   crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit     Crandall