Today I worked with the new DEG list from DESeq2 - comparing elevated (10˚C) and decreased (4˚C) temperature treatments. Annotated with uniprot-SP-GO and used DAVID to get enriched biological processes. Additionally, since it could be interesting to compare between oyster and crab projects, I annotated the list of differentially abundant...
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Submitted Thesis Chapters to Committee; Start working with New transcriptome
Today I submitted my two thesis chapters to my committee, and between now and June 2nd (my defense date), I’ll be doing more differential expression analyses with the new transcriptome (not filtered based on taxonomy). Plan for the work and what I’ve done so far detailed in post.
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Create annotated crab transcriptome BLAST `join` with uniprot SP GO
Worked a lot on the crab paper intro and bits of results and discussion here and there. I also made an annotated BLAST output from the crab transcriptome with the uniprot-SP-GO.sorted file.
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April 2020 Goals
Crab
finish paper
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Annotate 2019 infection status DEG list; short-term paper plan
Today I read a lot about crustacean immune systems and macropinocytosis. Notes in my crab paper. Additionally, SR and I met and chatted about short-term plan. I annotated the 2019 infection status DEG list. Details in post
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