Today I made a list of Trinity_IDs that are associated with the GOslim term “stress response” from the annotated crab transcriptome. I also clean up a few things in the project-crab/analyses repo… kind of.
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Sent samples to Genewiz
Today I shipped samples to Genewiz for individual RNAseq. Details in post.
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February Goals
Crab
Extract RNA from all remaining samples (Sam and Kaitlyn are going to keep helping, I think)
Prepare for qPCR on individual crab samples
Run qPCRs
Start working on crab paper
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Submitted 6 pooled crab samples to NWGC for QC
Today I submitted 6 pooled samples of Crab RNA to NWGC for QC. After they run QC, they’ll let us know what our sequencing options are.
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Extract RNA from 24 samples - Day 9, 12 infected, 12 uninfected; still need to run on Qubit
Today I extracted RNA from 24 samples. Day 9, 12 infected and 12 uninfected. I did not run them on the qubit yet. Will do later this week.
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