Today I tried out the Trizol LS Reagent extraction on three samples from Day 26. I used 1ul of each sample to run on the Qubit (RNA High Sensitivity), and all three samples were “out of range”. Images and more details in the post below:
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First Attempts- Trying new BLAST +2.81 with new taxid options
Today I’m trying to get started on the new BLAST +2.81 that has new databases and improved performance. This is pretty exciting because once I figure out how this works, I’ll be able to easily get taxonomy information like Order, Class, etc. I’m attempting what I believe is the first...
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Error 3 on Centrifuge- Couldn't finish Extraction with TriZol LS Reagent
Today I tried out the Trizol LS Reagent extraction, but the centrifuge in the 4˚C in FTR 213 had an “Err 3” message, which happens when there’s an error in the rotational speed measurement system (GitHub Issue #542). I’ll put a different centrifuge in the 4C and re-try the protocol...
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2015 Oysterseed DIA MSStats Figures Progress
Today I went through my R script for the RobertsLab/project-pacific.oyster-larvae project. I have a Volcano Plot and a Comparison Plot. I also did some data process plots: Profile Plots and QC Plots. I am working on updating the MS Stats protocol, and will include all the resources I used to...
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January 2019 Goals
2015 Oysterseed DIA
Finish data analysis
Edit paper with new methods, etc.
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