Yesterday and today I got some more 2015 DIA paper-related tasks done, including making a table that includes the proteins detected above a log2FC of 3.00 and below -3.00, and one that was annotated…
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RNeasy Kit Extraction with 12 samples - RNA in 1/12
Today I did the RNeasy Plus Micro Kit protocol (without the vacuum) on 12 samples (taken from the 24 that I did last time). Of the 12, only 1 had detectable RNA. The rest were all “Out of Range: TOO LOW”. Details in post below.
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Re-do MS Stats; DIA 2015 Oyster Updates
Today I got some more input from Emma regarding the reports to export from Skyline to analyze. I exported a new MS Stats report (the built-in one from Skyline) and also made the bioreplicates in the file accurate (details in post). Additionally, I exported a new report that includes information...
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April Goals
Crab Project
Identify methods of extracting RNA from low input
Test out more RNA extraction methods
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2015 DIA Oysterseed Paper Progress
A little bit of progress has been made with the DIA proteomics paper. I am aiming to have the methods section of the paper solidly done by our meeting this Wednesday at 2 pm.
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