Spoke with Sam the other day about everything we’ve tried in regards to extracting RNA from our crab hemolymph samples. He shared a lot of ideas of potential next steps for figuring out ….
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February Goals
2015 Oysterseed DIA
continue to work on the paper and figures
get feedback from people
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Trizol LS Reagent RNA extraction using Geoduck hemolymph and oyster tissue
Today I tried out the Trizol LS Reagent RNA extraction protocol on two geoduck (P. generosa) hemolymph and two oyster (C. virginica) tissue samples. (GitHub Issue #533)
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Qiagen RNeasy Micro Plus Kit with Geoduck hemolymph and oyster tissue
Today I also tried the Qiagen RNeasy Micro Plus Kit on geoduck (P. generosa) hemolymph (2 samples) and oyster (C. viriginica) tissue (2 samples).
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Trizol LS Reagent RNA Extraction Attempt
Today I tried the TrizolLS Reagent extraction again, but added a vortex (5s) step after the addition of chloroform to the samples. Throughout the extraction, the samples looked pretty similar to how they did during my last attempt, and the Qubit results were the same: “out of range”.
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