Today I checked on my finished BLAST on Mox with my assembled C. bairdi transcriptome and the nt taxonomy database. It finished after three-ish days, and the file was large. However, all of the taxonomy cells were “N/A”. I made a GitHub Issue, and got some input and am now...
[Read More]
Bioanalyzer Results - batch of cold, infected day 26
Today I ran the bioanalyzer on the batch of 8 samples from Day 26 (cold, infected) from Nov 21st. Results don’t look super great, and the ladder didn’t show up.
[Read More]
DIA analysis 2015 Cg seed continued
Today I am continuing my work in MS stats and working with the exported Skyline report. I updated the protocol to document what I have been doing to get where I am. I am in contact with Meena from MS stats, who is helping me with an error that keeps...
[Read More]
2015 C. gigas oysterseed DIA update
Today I worked on the DIA protocol and now have an exported report that I think I can start using with MS Stats. Still waiting on some input from Emma, but details are below:
[Read More]
DIA protocol, and Next Steps
Today I worked on documenting what I did (writing up a protocol of sorts) that is in progress. I am re-doing a couple steps just so that I’m ABSOLUTELY certain where files came from, and I’m putting all the new outputs in a brand-new directory so that it’s just all...
[Read More]