Today I extracted RNA from 10 samples from Day 26. These still had all three samples in the box, so they are all ###-1 tubes. The QUbit wouldn’t let me download the data from the USB. I’m not sure why, but luckily I wrote down, as I always do, the...
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Plan for R and Bioanalyzer results
Today I discussed with Sam about how to use R for making a better master file. We identified some short term and long term goals for me to work on (and get help with via GitHub Issues). I also ran the Bioanalyzer on Test3 (from when lyophilizer was used and...
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Extract RNA from non-lyophilized C. bairdi hemolymph
Today I extracted RNA from four practice samples using Tri-reagent, and without lyophilizing (lyophilizer is out of commission again for now).
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October Goals
Crab Project
Identify successful RNA extraction protocol
Get extracting RNA going so that we can plan to send some more samples for library prep and sequencing
Publish more DecaPod episodes (about 2 - one every two weeks)
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DecaPod Submitted to Apple, RNA Extraction plan for Mon/Tues
Today I submitted DecaPod RSS feed to Apple for approval to be published on iTunes! I also picked out some tubes for Tri-reagent RNA extraction without lyophilizing (it’s broken again) for Monday or Tuesday next week to get things moving again.
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