Received the Zymo RNA Clean and Concentrator Kit -5 yesterday. Plan is to concentrate my 6 pooled samples. I tested it out on some test pools I made quickly. Short answer: unclear if it worked because the initial qubit RNA HS readings of the test pools were both “TOO LOW”…...
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January 2020 Goals
Crab Concentrate the 6 new pooled crab samples (recieved that sample kit of the RNA Clean and Concentrator -5 kit from Zymo – have a lot of time tomorrow to work on this) Submit 6 new pooled samples (to NWGC because we have the quote from them) Work on new...
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April 2020 Goals
Oyster Paper
Finish solid draft by 4/2 or 4/3 at the latest and send out to lab for feedback
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Attempt at Concentrating Pooled (made from extra extracted RNA) Samples with the Zymo Microprep kit
So, it turns out my 6 pooled samples aren’t concentrated enough for NWGC to sequence. They are all at around 25ng/ul, but they need to be at least 50ng/ul. I called zymo yesterday to find out how best to do that. They recommended a kit (free sample coming next week)....
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December 2019 Goals
Crab:
Submit 6 new pooled samples to NWGC (waiting to hear back about how to submit samples (GitHub Issue: #798))
Work on assembling transcriptomes and moving forward with other 4 pooled libraries we currently have
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