Crab:
Submit 6 new pooled samples to NWGC (waiting to hear back about how to submit samples (GitHub Issue: #798))
Work on assembling transcriptomes and moving forward with other 4 pooled libraries we currently have
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Pooled 6 new samples for sequencing
Today I pooled samples for the 6 new libraries for sequencing. There is over 1000ng in each pooled sample, so everything is ready to go! Details in post.
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More RNA extracted from Day 9 - RNA in all
I extracted RNA from the same tubes that I worked with a lot the past couple of weeks on the bioanalyzer and Nanodrop and qubit because there ended up being not much left. RNA was in all samples. Details of extraction and next steps in post.
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GSS 2019 Preparations - Differential Expression between infected and uninfected
Today, I continued to prepare a presentation for GSS 2019 (this Thursday - my talk is at 1:30pm). I met with Steven and discussed a plan for results, and he shared with me the analyses he’s done during the past couple of days. Details of results and what I’ve done...
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Still no clear RNA bands on Bioanalyzer; Also working to get diff. exp. for GSS
Today I went through the DNase-free plan with 4 of the extracted crab samples. There was initially quite a bit of DNA, then after I used the Turbo DNA-free Kit, there was significantly less DNA. The Bioanalyzer still didn’t look super great… detail in post. Also, I’m working through kallisto...
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