Lab Notebook

Today I tried doing several things, none of them super successfully. First, I tried using Kallisto to get some quick differential expression results from the new bairdi RNA-seq data. It didn’t work, but I just need to look a little more into it. I didn’t spend too much time on... [Read More]

Today, Sam, Steven and I met to discuss a pooling plan for libraries (details in post). From that meeting it was decided that I should extract RNA from more day 12 samples, specfically from cold, warm, and infected and uninfected treatments. All 24 samples had RNA. An updated sample summary... [Read More]

Crab This coming week: Finish extracting RNA for pools by next week Select samples for 6 pools Day 9 infected Day 9 uninfected Day 12, cold, infected Day 12, cold, uninfected Day 12, warm, infected Day 12, warm, uninfected Run those samples for pooling on bioanalyzer; if they look good,... [Read More]

Today I extracted RNA from 24 more samples from Day 12. Mix of infection status and temperature exposures. There was RNA in 23 out of 24 samples. No obvious mistakes were made, and at the end of the post I provide an updated RNA sample summary in preparation for our... [Read More]

Today I did some more extractions, but moved on to day 12. I did a mix of all groupings (temperature and infection statuses). All extracted samples had detectable RNA! Details in post. Additionally, I’ll give a summary table of what all I have extracted so far. [Read More]