Today I did some more extractions, but moved on to day 12. I did a mix of all groupings (temperature and infection statuses). All extracted samples had detectable RNA! Details in post. Additionally, I’ll give a summary table of what all I have extracted so far.
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Second batch of 24 Crab hemolymph samples - RNA extracted and qubit results
Today I did another round of Day 9 infected (12) and uninfected (12) tanner crab hemolymph RNA extractions. I also ran 2ul of the eluted 15ul RNA on Qubit. Results and details in post.
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Crab RNA extractions with Qubit results
Today I ran 2ul of the 24 samples I extracted yesterday. 20 out of 24 had RNA. Details in post.
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Notes on protocol and plan for Crab Project extractions - use info from Sam's notebook posts
This post contains my notes on the Zymo Research Protocol for Quick-DNA/RNA Microprep kit, and Sam’s notebook post to create a protocol for extracting RNA. I also include my extraction plan for this next week as I try to get enough RNA to get two more libraries (Day 9 infected...
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October 2019 Goals
Crab
Work on paper
Pick samples accounting for temperature and infection status from master-qubit-sample.csv
Assemble transcriptomes, annotate etc. once new data arrives (should be any day now!)
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