Today I did another round of Day 9 infected (12) and uninfected (12) tanner crab hemolymph RNA extractions. I also ran 2ul of the eluted 15ul RNA on Qubit. Results and details in post.
[Read More]


Crab RNA extractions with Qubit results
Today I ran 2ul of the 24 samples I extracted yesterday. 20 out of 24 had RNA. Details in post.
[Read More]
Notes on protocol and plan for Crab Project extractions - use info from Sam's notebook posts
This post contains my notes on the Zymo Research Protocol for Quick-DNA/RNA Microprep kit, and Sam’s notebook post to create a protocol for extracting RNA. I also include my extraction plan for this next week as I try to get enough RNA to get two more libraries (Day 9 infected...
[Read More]
October 2019 Goals
Crab
Work on paper
Pick samples accounting for temperature and infection status from master-qubit-sample.csv
Assemble transcriptomes, annotate etc. once new data arrives (should be any day now!)
[Read More]
Oysterseed DIA - trying to characterize list of detected proteins in relation to _C. gigas_ proteome
I’m still working on wrapping up the results and discussion of this paper. Steven recommended I look into characterizing the list of detected proteins (2,808) in relation to the whole C. gigas proteome. Detailed in post:
[Read More]