Today I submitted 6 pooled samples of Crab RNA to NWGC for QC. After they run QC, they’ll let us know what our sequencing options are.
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Extract RNA from 24 samples - Day 9, 12 infected, 12 uninfected; still need to run on Qubit
Today I extracted RNA from 24 samples. Day 9, 12 infected and 12 uninfected. I did not run them on the qubit yet. Will do later this week.
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6 pooled Crab RNA samples - unsuccessful concentration with Zymo RNA Clean and Concentrator Kit -5
Today I tried to concentrate the 6 pooled crab RNA samples. Long story short, a lot of RNA was lost in the process and the “concetrated” samples are now well below the requirements for NWGC for RNAseq. Details in post. Next steps, I’m going to extract more RNA from samples...
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Attempt to concentrate test pools using Zymo RNA Clean and Concentrator Kit -5
Received the Zymo RNA Clean and Concentrator Kit -5 yesterday. Plan is to concentrate my 6 pooled samples. I tested it out on some test pools I made quickly. Short answer: unclear if it worked because the initial qubit RNA HS readings of the test pools were both “TOO LOW”…...
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January 2020 Goals
Crab Concentrate the 6 new pooled crab samples (recieved that sample kit of the RNA Clean and Concentrator -5 kit from Zymo – have a lot of time tomorrow to work on this) Submit 6 new pooled samples (to NWGC because we have the quote from them) Work on new...
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