Today I extracted RNA from 24 more samples from Day 12. Mix of infection status and temperature exposures. There was RNA in 23 out of 24 samples. No obvious mistakes were made, and at the end of the post I provide an updated RNA sample summary in preparation for our...
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Day 12 RNA extractions - RNA in all samples!
Today I did some more extractions, but moved on to day 12. I did a mix of all groupings (temperature and infection statuses). All extracted samples had detectable RNA! Details in post. Additionally, I’ll give a summary table of what all I have extracted so far.
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Second batch of 24 Crab hemolymph samples - RNA extracted and qubit results
Today I did another round of Day 9 infected (12) and uninfected (12) tanner crab hemolymph RNA extractions. I also ran 2ul of the eluted 15ul RNA on Qubit. Results and details in post.
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Crab RNA extractions with Qubit results
Today I ran 2ul of the 24 samples I extracted yesterday. 20 out of 24 had RNA. Details in post.
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Notes on protocol and plan for Crab Project extractions - use info from Sam's notebook posts
This post contains my notes on the Zymo Research Protocol for Quick-DNA/RNA Microprep kit, and Sam’s notebook post to create a protocol for extracting RNA. I also include my extraction plan for this next week as I try to get enough RNA to get two more libraries (Day 9 infected...
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