Today we had our first WSG meeting with the goal of having all of the projects wrapped up by April 17th.
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RNeasy Extraction Day 1 on 24 samples
Today I tried out the new plan for extracting RNA. It took quite a long time and none of the 24 samples had detectable RNA. Details in post.
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Bairdi hemolymph extraction plan (work in progress)
Here’s the general extraction plan after feedback from lab meeting today. I am planning on getting started on extractions next Tuesday (I’ll be done with end-of-quarter work and my online SCUBA class by then!). (Edited this post a lot since my original post this morning)
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2nd Half of March goals
I forgot to do March goals at the beginning of the month… so here’s what my goals are for the rest of the month! Classes are over this Friday, and I’m not going anywhere for the break, so I think I can get this done!
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RNeasy Kit Tube 516-2 starting sample testing
Today I extracted RNA from tube 516-2 in two different volumes: 10ul and 50ul. The tube only had about 62ul of sample slurry remaining, so I was only able to do 10ul and 50ul. I used the same protocol as what Steven and Shelly did last Friday. I saved the...
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