Today I edited and published S1E13 of DecaPod. In this episode I interview Madi Shipley (MS, SAFS) who works with Bering Sea Tanner crabs. I also extracted RNA from 8 samples (all Cold, infected, Day 26) using Qiagen RNeasy and definitely have enough RNA for a pool!
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RNeasy Test Bioanalyzer Results
Today I ran the two samples from Day 26 that I extracted using RNeasy Kit on the Bioanalzyer. The results look pretty good and we can now make a library and see how it goes.
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Submitted GSS poster, and my goals for Th and F
Today at 4:25pm, I submitted my poster to be printed for GSS, which is tomorrow… Steven helped a lot with picking out what information would be interesting to include. My goals for Thursday and Friday include both the Crab Project and the 2015 Oysterseed Project.
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BLASTn with Blue Crab and Prep for GSS Poster
Today I BLASTn -ed Blue crab transcriptome with the assembled C. bairdi transcriptome on Mox. I also finished up some more things in my BLAST to GO GO-slim notebook with uniprot/sprot. Additionally, I got some input from Steven on how best to create a poster for GSS (which is this...
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BLAST on Hummingbird and Transrate
Today I attempted to take my BLAST output on Hummingbird and work through this blast-2-slim notebook of Steven’s. I had gone through this before using Cgigas. I got stuck today at the part where you !join _blast-GO-unfolded.sorted with GO-GOslim.sorted. Will ask tomorrow. Additionally, today I worked on using transrate to...
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