Today I ran the bioanalyzer on the batch of 8 samples from Day 26 (cold, infected) from Nov 21st. Results don’t look super great, and the ladder didn’t show up.
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DIA analysis 2015 Cg seed continued
Today I am continuing my work in MS stats and working with the exported Skyline report. I updated the protocol to document what I have been doing to get where I am. I am in contact with Meena from MS stats, who is helping me with an error that keeps...
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2015 C. gigas oysterseed DIA update
Today I worked on the DIA protocol and now have an exported report that I think I can start using with MS Stats. Still waiting on some input from Emma, but details are below:
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DIA protocol, and Next Steps
Today I worked on documenting what I did (writing up a protocol of sorts) that is in progress. I am re-doing a couple steps just so that I’m ABSOLUTELY certain where files came from, and I’m putting all the new outputs in a brand-new directory so that it’s just all...
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2015 Oysterseed Plan and Crab Meeting Notes
Crab Meeting today: shared current status of what we’ve got (FISH546 grace-Cbairdi-transcriptome). Additionally, we had a proteomics meeting today and I got some input on next steps for the DIA: set some stringent thresholds, MS Stats, make some tables, etc. I will make a separate post on what I did...
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