This post contains a slightly modified RNeasy Kit protocol. I want to use the Qiagen RNeasy Micro Plus Kit on 4 crab hemolymph pellet samples and the four corresponding supernatant samples (GitHub Issue 577). The goal is to see if there is any RNA in the supernatant samples and if...
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Notes, Thoughts, and Ideas on RNA isolation (and thoughts from Sam)
Spoke with Sam the other day about everything we’ve tried in regards to extracting RNA from our crab hemolymph samples. He shared a lot of ideas of potential next steps for figuring out ….
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February Goals
2015 Oysterseed DIA
continue to work on the paper and figures
get feedback from people
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Trizol LS Reagent RNA extraction using Geoduck hemolymph and oyster tissue
Today I tried out the Trizol LS Reagent RNA extraction protocol on two geoduck (P. generosa) hemolymph and two oyster (C. virginica) tissue samples. (GitHub Issue #533)
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Qiagen RNeasy Micro Plus Kit with Geoduck hemolymph and oyster tissue
Today I also tried the Qiagen RNeasy Micro Plus Kit on geoduck (P. generosa) hemolymph (2 samples) and oyster (C. viriginica) tissue (2 samples).
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