I prep for RNA isolation and understanding the protocol, and continue with the 2015 Oysterseed DIA.
[Read More]
Podcast thoughts and Crab hemoolymph samples
Initial info on making a Podcast from Audacity workshop at UW and Crab Hemolymph samples next steps - testing out the RNA isolation protocol.
[Read More]
Preliminary Crab Sample Selection for Seq
Today I chose some crab samples for RNA extraction. I made a chart with the tube numbers for the samples. All crabs are immature males. Due to a massive die-off in the warm temperature treatment groups, we unfortunately only have two infected and one uninfected sample per the final two...
[Read More]
qPCR Machine arrival and training and Crab data and DIA updates
New qPCR machine arrived, training with Brian, and preliminary crab hemolymph sample picking for RNA isolation.
[Read More]
DIA Next Steps and Crab Data Spreadsheet
I posted the crab data from Pam to Owl, and I hear back from Emma in regards to my error rates.
[Read More]